Other

Part:BBa_M36544:Design

Designed by: Danielle Fraga   Group: Stanford BIOE44 - S11   (2013-10-25)


cAMP-activated global transcriptional regulator CRP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 373
    Illegal PstI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 373
    Illegal PstI site found at 534
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 373
    Illegal PstI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 373
    Illegal PstI site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was a one codon overlap between the RBS and the protein, so we removed that repeat. We had to find the specific regulatory protein that binds to cAMP.


Source

http://www.uniprot.org/uniprot/P0ACJ8

References